Procedure |
Preparation |
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Dissolve 3 g sodium chloride and 10 ml detergent with 100ml distilled water. Cut the onion into small pieces and grind in the above detergent-salt mixture. |
High temperature incubation |
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Incubate the mixture in a 60℃ water bath for 15 minutes with gentle stirring. |
Ice bath incubation |
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Incubate the mixture in ice bath for 5 minutes, with gentle stirring. |
Blending |
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Blend the mixture in a blender for 5 seconds, and filter the mixture. |
Filtration |
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Collect the filtrate with a test tube, add 4 drops of protease into 6 ml filtrate. |
Alcohol precipitation |
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Slowly add the ice-cold absolute alcohol at the side of the test tube onto the filtrate's surface. Use a dropper to remove the intermediate milky layer -- the DNA fraction, and test the pH of it with universal indicator. |
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